{"id":118856,"date":"2026-09-11T21:49:27","date_gmt":"2026-09-11T19:49:27","guid":{"rendered":"https:\/\/nilu.gnist.dev\/publikasjoner\/characterizing-cytotoxic-and-estrogenic-activity-of-arctic-char-tissue-extracts-in-primary-arctic-char-hepatocytes\/"},"modified":"2026-09-11T21:49:27","modified_gmt":"2026-09-11T19:49:27","slug":"characterizing-cytotoxic-and-estrogenic-activity-of-arctic-char-tissue-extracts-in-primary-arctic-char-hepatocytes","status":"publish","type":"nva_publication","link":"https:\/\/nilu.gnist.dev\/en\/publications\/characterizing-cytotoxic-and-estrogenic-activity-of-arctic-char-tissue-extracts-in-primary-arctic-char-hepatocytes\/","title":{"rendered":"Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes"},"content":{"rendered":"<p class=\"wp-block-paragraph\">Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasj\u00f8en at Bj\u00f8rn\u00f8ya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char (Salvelinus alpinus) from this lake compared to fish from other lakes on Bj\u00f8rn\u00f8ya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and\/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasj\u00f8en with those from less contaminated Lake Laksvatn at Bj\u00f8rn\u00f8ya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasj\u00f8en fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasj\u00f8en fish reduced in vitro cell viability at a concentration ratio of 0.03\u20131 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasj\u00f8en fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasj\u00f8en F3 extracts, it remains to be determined which compounds were inducing estrogenic effects.<\/p>\n","protected":false},"template":"","meta":{"_acf_changed":false,"_searchwp_excluded":"","_id":"","_status":"PUBLISHED","_sync_date":"2026-09-11 14:48:28","footnotes":""},"nva_tax_category":[1099],"class_list":["post-118856","nva_publication","type-nva_publication","status-publish","hentry","nva_tax_category-scientific-journal-publication"],"acf":[],"_links":{"self":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication\/118856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication"}],"about":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/types\/nva_publication"}],"version-history":[{"count":0,"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication\/118856\/revisions"}],"wp:attachment":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/media?parent=118856"}],"wp:term":[{"taxonomy":"nva_tax_category","embeddable":true,"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_tax_category?post=118856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}