{"id":71821,"date":"2026-10-27T22:56:58","date_gmt":"2026-10-27T21:56:58","guid":{"rendered":"https:\/\/nilu.gnist.dev\/publikasjoner\/metabolism-of-small-antimicrobial-%ce%b222-amino-acid-derivatives-by-murine-liver-microsomes\/"},"modified":"2026-10-27T22:56:58","modified_gmt":"2026-10-27T21:56:58","slug":"metabolism-of-small-antimicrobial-%ce%b222-amino-acid-derivatives-by-murine-liver-microsomes","status":"publish","type":"nva_publication","link":"https:\/\/nilu.gnist.dev\/en\/publications\/metabolism-of-small-antimicrobial-%ce%b222-amino-acid-derivatives-by-murine-liver-microsomes\/","title":{"rendered":"Metabolism of small antimicrobial \u03b22,2-amino acid derivatives by murine liver microsomes"},"content":{"rendered":"<p class=\"wp-block-paragraph\">We have investigated the in vitro metabolism of three small antimicrobial \u03b22,2-amino acid derivatives (M w &lt; 500) that are highly potent against methicillin resistant Staphylococcus aureus, and are among the first compounds designed from small cationic antimicrobial peptides with potential for oral administration. The \u03b22,2-amino acid derivatives are virtually completely resistant against degradation by proteases, and to further explore their drug potential, we have investigated the hepatic Phase I metabolism of this class of antimicrobial compounds. The \u03b22,2-amino acid derivatives were incubated with murine liver microsomes and the metabolites analyzed semi-quantitatively by HPLC\u2013MS and qualitatively by ultra performance liquid chromatography coupled to a tandem mass spectrometer which enabled identification of the metabolites by careful interpretation of the collision activated dissociation spectra. The study shows that sterically hindered \u03b22,2-amino acid derivatives that otherwise are stable against proteolytic degradation underwent Phase I metabolism and were oxidized to a number of different metabolites depending on the structure of the \u03b22,2-amino acid side-chains. \n\n<\/p>\n","protected":false},"template":"","meta":{"_acf_changed":false,"_searchwp_excluded":"","_id":"","_status":"PUBLISHED","_sync_date":"2026-10-27 22:52:27","footnotes":""},"nva_tax_category":[927],"class_list":["post-71821","nva_publication","type-nva_publication","status-publish","hentry","nva_tax_category-scientific-journal-publication"],"acf":[],"_links":{"self":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication\/71821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication"}],"about":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/types\/nva_publication"}],"version-history":[{"count":0,"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_publication\/71821\/revisions"}],"wp:attachment":[{"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/media?parent=71821"}],"wp:term":[{"taxonomy":"nva_tax_category","embeddable":true,"href":"https:\/\/nilu.gnist.dev\/en\/wp-json\/wp\/v2\/nva_tax_category?post=71821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}