Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Peter Møller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milic, Goran Gajski, Solange Costa, João Paulo Teixeira, Cristiana Costa Pereira, Maria Dusinska, Roger Godschalk, Gunnar Brunborg, Kristine Bjerve Gutzkow, Lisa Giovannelli, Marcus S. Cooke, Elke Richling, Blanca Laffon, Vanessa Valdiglesias, Nursen Basaran, Cristian Del Bo, Bojana Zegura, Matjaz Novak, Helga Stopper, Pavel Vodicka, Sona Vodenkova, Vanessa Moraes de Andrade, Monika Srámková, Alena Gábelová, Andrew Richard Collins, Sabine A.S. Langie

Publikasjonsdetaljer

Publikasjonstype: Vitenskapelig artikkel

Tidsskrift: Nature Protocols

Volum: 15

Utgave: 15

Sider: 3817-3826

Original versjon/DOI: https://doi.org/10.1038/s41596-020-0398-1

NVA: https://hdl.handle.net/11250/2688559

Sammendrag:

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.